Zea mays Leaf Dev Li et al, 2010

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Data set to be added in Release #21 spring 2014, based on Li et al (2010)

Background:

The study authors analyzed the maize leaf transcriptome using Illumina sequencing. They mapped more than 120 million reads to define gene structure and alternative splicing events and to quantify transcript abundance along a leaf developmental gradient and in mature bundle sheath and mesophyll cells.

File:Figure 1 (Li et al, 2010).png

  • The samples were mapped to PO ids representing the plant structures and plant growth stages used.
  • Data is from:
    • Supplementary Table 2: qRT-PCR analysis of a subset of genes in the 4 stages.
    • Supplementary Table 4: List of genes exhibiting differential expression between bundle sheath and mesophyll cells.


PO anatomy and growth stage mapping table:

Tissue Paper description PO Anatomy term PO ID PO Development stage term PO ID Comments
Leaf base1 cm above the leaf #3 liguleleaf lamina basePO:0008019vascular leaf initiation stagePO:0001051represents sink tissue
""vascular leafPO:0009025LP.03 three leaves visible stagePO:0007106
Transitional zone1 cm below the leaf #2 ligulevascular leafPO:0009025vascular leaf differentiation stagePO:0025574Represents the sink-source transition
""""LP.03 three leaves visible stagePO:0007106
Maturing zone4 cm above the leaf #2 ligule vascular leafPO:0009025vascular leaf expansion stagePO:0001052 Represents a maturing leaf, this position is between the leaf base and leaf tip.
""central zone of the leaf laminaPO:0008020LP.03 three leaves visible stagePO:0007106
Leaf Tiprepresents a mature leafleaf tipPO:0025142vascular leaf mature stage PO:0001053
""leaf lamina tipPO:0025589LP.03 three leaves visible stagePO:0007106represents a mature leaf
""vascular leafPO:0009025""
mesophyll cellsMain site of carbon fixation particularly in C4 grasses.leaf mesophyll cellPO:0004006vascular leaf mature stage PO:0001053
""leaf lamina tipPO:0025589LP.03 three leaves visible stagePO:0007106
bundle sheath cellsMain site of Calvin cycle particularly in C4 grassesbundle sheath cellPO:0025541vascular leaf mature stage PO:0001053
""leaf lamina tipPO:0025589LP.03 three leaves visible stagePO:0007106


Li, et al. “The Developmental Dynamics of the Maize Leaf Transcriptome.” Nature Genetics 42, no. 12 (December 2010): 1060–1067. doi:10.1038/ng.703. link to paper: http://www.nature.com/ng/journal/v42/n12/abs/ng.703.html#supplementary-information

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